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RT-qPCR reaction conditions.
| Procedure | Temperature (°C) | Duration |
|---|---|---|
| Pre-denaturation 35 cycles | 95 | 3 min |
| Denaturation | 95 | 10 sec |
| Annealing | 56.8, 62.3 and 57.5 for β-actin, TLR4 and TLR2 respectively | 30 sec |
| Elongation | 65 | 1 min |
| Renaturation | 72 | 5 min |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TLR 4, Toll-like receptor 4; TLR 2, Toll-like receptor 2.
