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Western blot and densitometric analysis of Wnt3a protein expression in the striated muscle complex of normal and ARM model rats at various embryonic developmental stages. Wnt3a expression was normalized to β-actin expression in the developing SMC of normal and ARMs rat embryos at E17, E19 and E21. *P<0.05 vs. corresponding control. ARM, anorectal malformation; E, embryonic day.
