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Distribution of embryos in the various age and treatment groups.
| Normal group | ARMs group | ||||||
|---|---|---|---|---|---|---|---|
| Age group | IHC | WB | RT-qPCR | IHC | WB | RT-qPCR | |
| E17 | 17 | 20 | 21 | 21 | 26 | 25 | |
| E19 | 12 | 18 | 16 | 18 | 24 | 23 | |
| E21 | 11 | 16 | 12 | 17 | 22 | 22 | |
| Total | 40 | 54 | 49 | 56 | 72 | 70 | |
ARMs, anorectal malformations; E, embryonic day; IHC, immunohistochemical staining; WB, Western blot.
