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. 2017 Feb 13;15(4):1722–1726. doi: 10.3892/mmr.2017.6199

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Copyright: © Ota et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression of RhoGDIβ during the differentiation of THP-1 cells to macrophages. For the sampling of differentiation-induced cells, unattached dead and apoptotic cells were removed. The cell lysate was immunoblotted with an antibody (sc-6047), which recognizes full-length RhoGDIβ and Δ19-RhoGDIβ, and an antibody (97A1015), which specifically recognizes Δ19-RhoGDIβ. α-tubulin was stained with B-5-1-2 antibody as a loading control. Similar results were obtained for three independent experiments. Representative results are shown. RhoGDIβ, Rho GDP-dissociation inhibitor β.