Figure 3.

Inhibition of RA FLS cell proliferation by TRIM3 overexpression. (A) Results of CCK-8 assays following infection of pLVX-puro or pLVX-puro-TRIM3 lentivirus into RA FLS cells. Proliferation was significantly suppressed by pLVX-puro-TRIM3 viral transduction. (B) Expression of TRIM3, cyclin D1, PCNA, p53 and p21 was evaluated by western blot analysis at 48 h after viral transduction. Data are based on three independent experiments, and shown as mean ± SD. *P<0.05, **P<0.01, ***P<0.001. RA, rheumatoid arthritis; FLS, fibroblast-like synoviocytes; TRIM3, tripartite motif-containing protein 3; CCK-8, Cell Counting Kit-8.