Figure 4.

LXA₄-stimulated Nrf2 translocation was dependent on PPARγ activation. (A) HK-2 cells were pretreated with 10 nM LXA₄ for 12 h and transfected with PPARγ siRNA, and then exposed to H/R. The cytosolic or nuclear Nrf2 proteins were determined using western blotting. Nuclear Nrf2 was expressed as Nrf2/tubulin ratio and the cytosolic Nrf2 was expressed as Nrf2/β-actin ratio for each sample. Data are presented as the mean ± standard deviation of 5 independent experiments. *P<0.05 vs. control group. (B) Nrf2 translocation was assessed using fluorescence microscopy (magnification ×400) and cells were pretreated with LXA₄ and exposed to H/R. Nrf2, nuclear factor E2-related factor 2; PPARγ, peroxisome proliferator-activated receptor-γ; siRNA, small interfering RNA; H/R, hypoxia/reoxygenation; LXA₄, lipoxin A₄; FITC, fluorescein isothiocyanate.