Figure 4.

Function of chimeric TCRs in PBMCs. (A) Human PBMCs expressing either wtTCR or modified TCR variants were co-cultured with different tumor cell lines for 24 h, and concentrations of IFN-γ secreted into the co-culture supernatant were measured using ELISA. (B) Specific cytotoxicity of tumor cell lines. The human PBMCs expressing the wtTCR, TCR∆IgC, TCR∆cp+tm+ic or TCR∆C transgenes, or without a TCR transgene were co-cultured with CAM-labeled tumor cell lines at the indicated E:T ratios for 4 h, following which specific lysis was calculated. Results represent the average of three independent experiments, performed with three donors. *P<0.05, **P<0.01, ***P<0.001). TCR, T cell receptor; PBMCs, peripheral blood mononuclear cells; wt, wild-type; Ig, immunoglobulin-like; cp+tm+ic, connecting peptide, transmembrane and intracellular; C, complete constant; CAM, calcein AM; IFN-γ, interferon-γ; HLA, human leukocyte antigen; E:T, effector to target cell; ns, non-significant.