Table III.
Primers for amplifying TCRβ∆IgC-EYFP and TCRα∆IgC-ECFP fusion genes (regular polymerase chain reaction (PCR) and SOE-PCR).
| Gene name | Primer name | Direction | Primer sequence |
|---|---|---|---|
| TRBV | P1 | Forward | ATAGCTAGCGCCACCATGGGCTGCAGGCTGCTCTG |
| P2 | Reverse | ACATCTGCATCAAGTTGTTTCTCCAGTACGGTCAGCCT | |
| TRGC | P3 | Forward | AGGCTGACCGTACTGGAGAAACAACTTGATGCAGATGT |
| P4 | Reverse | CGGAGGTGAAGCCACAGTCTGTCTTTATTGGAGGAAAG | |
| TRBCm | P5 | Forward | CTTTCCTCCAATAAAGACAGACTGTGGCTTCACCTCCG |
| P6 | Reverse | CTCGCCCTTGCTCACCATGCCTCTGGAATCCTTTCT | |
| EYFP | P7 | Forward | AGAAAGGATTCCAGAGGCATGGTGAGCAAGGGCGAG |
| P8 | Reverse | CGGCGTCGACTTACTTGTACAGCTCGTC | |
| TRAV | X1 | Forward | ACGCCACAACCTTGGCCACCATGATATCCTTGAGAGTT |
| X2 | Reverse | GGTTTGGTATGAGGCTGACTATTTGGTTTTACTGTCAGTCTGG | |
| TRDC | X3 | Forward | CCAGACTGACAGTAAAACCAAATAGTCAGCCTCATACCAAACC |
| X4 | Reverse | GCTTGACATCACAGGAACTTTCTGTAGAATCTGTCTTCACTTC | |
| TRACm | X5 | Forward | GAAGTGAAGACAGATTCTACAGAAAGTTCCTGTGATGTCAAGC |
| X6 | Reverse | CTCCTCGCCCTTGCTCACCATGCTGGACCACAGCCGCAGC | |
| ECFP | X7 | Forward | GCTGCGGCTGTGGTCCAGCATGGTGAGCAAGGGCGAGGAG |
| X8 | Reverse | AGTGCGGCCGCTTACTTGTACAGCTCGTCCAT |
Restriction enzyme sites are underlined. SOE-PCR, splicing by overlap extension-polymerase chain reaction.