Table V.
Primers for amplifying TCRβ∆C-EYFP and TCRα∆C-ECFP fusion genes (regular polymerase chain reaction (PCR) and SOE-PCR).
| Gene | Primer name | Direction | Primer sequence |
|---|---|---|---|
| TRBV | P1 | Forward | ATAGCTAGCGCCACCATGGGCTGCAGGCTGCTCTG |
| P2 | Reverse | ACATCTGCATCAAGTTGTTTCTCCAGTACGGTCAGCCT | |
| TRGC+GCm | P3 | Forward | AGGCTGACCGTACTGGAGAAACAACTTGATGCAGATGT |
| O4 | Reverse | CCTCGCCCTTGCTCACCATTGATTTCTCTCCATTGCAG | |
| EYFP | O5 | Forward | CTGCAATGGAGAGAAATCAATGGTGAGCAAGGGC |
| P8 | Reverse | CGGCGTCGACTTACTTGTACAGCTCGTC | |
| TRAV | X1 | Forward | ACGCCACAACCTTGGCCACCATGATATCCTTGAGAGTT |
| X2 | Reverse | GGTTTGGTATGAGGCTGACTATTTGGTTTTACTGTCAGTCTGG | |
| TRDC+DCm | X3 | Forward | CCAGACTGACAGTAAAACCAAATAGTCAGCCTCATACCAAACC |
| H4 | Reverse | CCTCGCCCTTGCTCACCATCAAGAAAAATAACTTGGCAGT | |
| ECFP | H5 | Forward | ACTGCCAAGTTATTTTTCTTGATGGTGAGCAAGGGCGAGG |
| X8 | Reverse | AGTGCGGCCGCTTACTTGTACAGCTCGTCCAT |
Restriction enzyme sites are underlined. SOE-PCR, splicing by overlap extension-polymerase chain reaction.