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. 2016 Aug 22;8(1):366–370. doi: 10.1039/c6sc01831a

Table 1. Reduction of Cys and Lys disulfide catabolites using dithiothreitol (DTT) and glutathione (GSH).

Metabolite Site-specific # Me grps a % remaining b (DTT, 15 m) % remaining c (GSH, 24 h)
Cys-DM1 (4) Yes 0 67 16
Lys-SPDB-DM1 (6) No 0 87 62
Cys-DM3 (5) Yes 1 97 73
Lys-SPDB-DM3 (7) No 1 98 98
Lys-SPDB-DM4 (8) No 2 100 99

aNumber of methyl groups on adjacent atom to disulfide.

b15 µM metabolite, 50 µM DTT.

c15 µM metabolite, 50 µM GSH.