Table 1. Reduction of Cys and Lys disulfide catabolites using dithiothreitol (DTT) and glutathione (GSH).
Metabolite | Site-specific | # Me grps a | % remaining b (DTT, 15 m) | % remaining c (GSH, 24 h) |
Cys-DM1 (4) | Yes | 0 | 67 | 16 |
Lys-SPDB-DM1 (6) | No | 0 | 87 | 62 |
Cys-DM3 (5) | Yes | 1 | 97 | 73 |
Lys-SPDB-DM3 (7) | No | 1 | 98 | 98 |
Lys-SPDB-DM4 (8) | No | 2 | 100 | 99 |
aNumber of methyl groups on adjacent atom to disulfide.
b15 µM metabolite, 50 µM DTT.
c15 µM metabolite, 50 µM GSH.