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Two repetitions were performed, in which “m” represents vp9-99-2226-1 mutant, “WT” represents its wild-type counterpart, and “B73” represents the maize inbred line B73. Exons 1–2 represent the PCR products spanning the first and the second exons; the other names are indicated in the same manner, and GAPDH served as an internal control gene.
