. 2017 Mar 10;13(3):e1006669. doi: 10.1371/journal.pgen.1006669

Table 2. Bacterial strains used in this work.

Strain	Genotype	Phenotype/use	Source
E. coli DH5α	supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1 relA1	Ordinary cloning	[35]
E. coli BL21 (DE3)	F^- ompT gal dcm lon HsdSB (r_B^-m_B^-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])	Protein overexpression and purification	[36]
T. thermophilus strains
HB27wt	ATCC BAA-163/DSM7039	Wt	Y. Koyama
NAR1	[pTT27::nar]	wt. NCE, partial denitrifer	[37]
HB8	ATCC 27634	ICEth1-less	Y. Koyama
HB27^EC	HB27 ago::agoISTth7	enhanced competence	This work
Wt^Cm		HB27 Cm^r	This work
Δago	HB27 Δago	Argonaute less	[38]
ΔpilA4	HB27^EC ΔpilA4	Non competent	[16]
ΔpilA4::hyg	HB27^EC ΔTTC0858::hyg	Non competent	[16]
ΔtdtA	HB27^EC ΔTTC1879	Deficient in DNA donation	This work
tdtA::pk	HB27^ECTTC1879::pK18	Km^r Deficient in DNA donation.	This work
ΔtdtA::kat	HB27^EC ΔTTC1879::kat	Km^r. Deficient in DNA donation.	This work
ΔtdtA::hyg	HB27^EC ΔTTC1879::hyg,	Hyg^r. Impaired in DNA donation.	This work
tdtA::kat pilA4	HB27^EC ΔTTC1879::kat, ΔpilA4	Km^r. Deficient in DNA donation and transformation.	This work
Wt^H	HB27^ECΔTTC0313::hyg	Hyg^r	[16]
Nar tdtA::kat	NAR1 [ΔTTC1879::kat]	Nitrate respiring. Km^r	This work
Nar TTC::hyg	NAR1 [ΔTTC0313::hyg]	Nitrate respiring. Hyg^r	[16]
gdh::kat	HB27 [ΔTTC1211::kat]	Km^r	[37]
HB8 hyg	HB8 [ΔTTC0313::hyg]	ICEth1-less. Hyg^r	This work
HB8 Cm^r	HB8 spontaneous chloramphenicol resistant	ICEth1-less. Cm^r	This work
HB8::ICEth1::kat, Hyg^r	HB8 [ΔTTC0313::hyg], ICEth1::pK18	ICEth1, Km^r, Hyg^r	This work
tdtAYFPph	HB27^EC [TTC1879-sYFP:pH118]	Hyg^r. Single copy expression of TdtA fused to sYFP	This work
ΔtdtA, ΔpilA4 PMH	HB27^EC ΔTTC1879::kat, ΔpilA4 [pMH::TTC1879]	Complementation of TdtA	This work
ΔtdtA, ΔpilA4 PMHY	HB27^EC ΔTTC1879::kat, ΔpilA4 [pMH::TTC1879::sYFP]	Complementation of TdtA with TdtA-sYFP	This work
ICEth13’kat	HB27 TTC1880-TTC1881::pK18	Intergenic tag (TTC1880-TTC1881) of kanamycin marker.	This work
ΔTh111II::kat, ΔpilA4	HB27^EC ΔTTC1877::kat	Km^r. Impaired in DNA donation and transformation	This work
ΔnurA::kat, ΔpilA4	HB27^EC ΔTTC1878::kat	Km^r. Impaired in DNA donation and transformation	This work
ΔTTC0147::kat	HB27^EC ΔTTC0147::kat	Km^r. Affected in stress survival	This work
ΔTTC1430::kat	HB27^EC ΔTTC1430::kat	Km^r. Affected in stress survival	This work
ΔTTC1844::kat	HB27^EC ΔTTC1844::kat	Km^r. Affected in stress survival and transformation	[16]
Δgdh::kat	HB27 ΔTTC1211::kat	Km^r	[16]
ΔpilA4::kat	HB27 ΔTTC0858::kat	Km^r. Impaired in transformation	[16]
TTC1017::kat	HB27 ΔTTC1017::kat	Km^r. Impaired in transformation	[16]
TTC1622:kat	HB27 ΔTTC1622::kat	Km^r. Impaired in transformation	[39]
TTC854::kat	HB27 ΔTTC0854::kat	Km^r	[40]
TTC1621::kat	HB27 ΔTTC1621::kat	Km^r	[39]
TTC857::kat	HB27 ΔTTC0857::kat	Km^r	[40]
TTC0856::kat	HB27 ΔTTC0856::kat	Km^r	[40]
TTC1716::kat	HB27 ΔTTC1716::kat	Km^r	[40]
TTTC1603::kat	HB27 ΔTTC1603::kat	Km^r	[39]
TTC1415::kat	HB27 ΔTTC1415::kat	Km^r	[16]
TTC0638::kat	HB27 ΔTTC0638::kat	Km^r	[16]
TTC0893::kat	HB27 ΔTTC0893::kat	Km^r	[16]
TTC1425::kat	HB27 ΔTTC1425::kat	Km^r	This work
TTC1429::kat	HB27 ΔTTC1429::kat	Km^r	This work
TTC0474::kat	HB27 ΔTTC0474::kat	Km^r	This work
TTC0656::kat	HB27 ΔTTC0656::kat	Km^r	This work
TTC1026::kat	HB27 ΔTTC1026::kat	Km^r	This work
TTC1878::kat	HB27 ΔTTC1878::kat	Km^r	This work
TTC1877::kat	HB27 ΔTTC1877::kat	Km^r	This work
TTC1380::kat	HB27 ΔTTC1380::kat	Km^r	This work
TTC1013::kat	HB27 ΔTTC1013::kat	Km^r	[40]
TTC1873::kat	HB27 ΔTTC1873::kat	Km^r	This work
TTC440::kat	HB27 ΔTTC0440::kat	Km^r	[40]
TTC1695::kat	HB27 ΔTTC1695::kat	Km^r	This work
TTC1468::kat	HB27 ΔTTC1468::kat	Km^r	This work
TTC1839::kat	HB27 ΔTTC1839::kat	Km^r	This work
TTC1836::kat	HB27 ΔTTC1836::kat	Km^r	This work
TTC1876::kat	HB27 ΔTTC1876::kat	Km^r	This work
TTC1466::kat	HB27 ΔTTC1466::kat	Km^r	This work
TTC922::kat	HB27 ΔTTC0922::kat	Km^r	[16]
TTC1955::kat	HB27 ΔTTC1955::kat	Km^r	This work
TTC1721::kat	HB27 ΔTTC1721::kat	Km^r	This work
TTC1486::kat	HB27 ΔTTC1486::kat	Km^r	This work
TTC1657::kat	HB27 ΔTTC1657::kat	Km^r	This work
TTC657::kat	HB27 ΔTTC0657::kat	Km^r	This work
TTC1880::kat	HB27 ΔTTC1880::kat	Km^r	This work
TTC145::kat	HB27 ΔTTC0145::kat	Km^r	This work
TTC1014::kat	HB27 ΔTTC1014::kat	Km^r	[40]
TTC1018::kat	HB27 ΔTTC1018::kat	Km^r	[40]
TTC1844::kat	HB27 ΔTTC1844::kat	Km^r	[16]