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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Diabetes. 2017 Jan 13;66(4):1041–1051. doi: 10.2337/db15-1570

Figure 3.

Figure 3

Increased levels of endogenous RA are detected in tailbuds of E9 embryos of diabetic mice using a RA reporter cell line. A: Image illustrates the caudal-most portion of the tailbud (boundary marked by the dotted line) as excised for detection of bioactive RA. B: Percentage of tailbuds excised from embryos of ND and MD mice that have different numbers of positively stained cells induced in the RA reporter cell line (n = 19 for ND and 22 for MD from three and four litters, respectively). C: Representative images of excised tailbuds that were placed directly on the RA reporter cells and have different numbers of stained cells induced in the RA reporter cell line. The amount of stained cells is categorized as (-), (+), (++) and (+++) as defined in B.