Fig. 1.

Experimental protocol.

Mice were assigned to five different groups (n = 5–6). Four groups were ovariectomized (OVX), and one group was sham operated and remained untreated for 4 weeks after the operation. Four weeks after surgery, treatment with anti-RANKL mAb (5 mg/kg s.c., once at 4 weeks after OVX), PTH (80 μg/kg/day s.c., daily injection for 4 weeks), or Ab plus PTH was started in the OVX groups. All mice were sacrificed 8 weeks after operation.