Fig. 6.

MTA stimulates Atf6 binding to promoter region of Bglap gene. The cells were stimulated by MTA for 4 days. ChIP assays were performed. Cell lysates were incubated with non-immune IgG control or ATF6 antibodies. (A) PCR amplification of Bglap gene promoters was performed using immunoprecipitated and non-immunoprecipitated (input) DNA by using PCR thermal cycler. (B) A quantitative study of immunoprecipitated DNA was performed by using real-time PCR. Data represent the means ± SEM (n = 3). Statistical significance of differences between MTA treated group and corresponding time-matched vehicle control is indicated by ***P < 0.001.