Fig. 7.

Forced expression of active Atf6 (p50Atf6) induces Bglap gene expression. The cells were transiently transfected with a p3 × FLAGp50CMV10 or mock (p3 × FLAGCMV10) in MC3T3-E1 cells and cultured for 2 days in osteogenic medium. (A) Protein level of Atf6 was analyzed by western blotting. (B) A quantitative study of intensity was performed by Molecular Imager FX (Bio-Rad). (C) Bglap (osteocalcin) mRNA expression was determined by real-time RT-PCR. Data represent the means ± SEM (n = 3). ***, P < 0.001.