Fig. 8.

Knockdown of Atf6 gene expression abolishes Bglap mRNA expression and mineralization by MTA. Stable clones of Atf6 shRNA were incubated with (+) or without (−) Dox and/or MTA for 16 days: MTA, 2.5 μg/mL; Dox, 1.0 μg/mL. (A) Whole-cell lysates were subjected to immunoblotting with anti-Atf6 and GAPDH antibodies on day 12 in culture. (B) A quantitative study for the intensity was performed by Molecular Imager FX (Bio-Rad). (C) Bglap mRNA expression was decreased by Atf6 shRNA. On day 12 in culture, total RNA was extracted from the cells, reverse-transcribed, and subjected to qPCR analysis. Data represent the means ± SEM (n = 3). ***, P < 0.001; NS, not significant.