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Typical DLS regularization fits for 30 nm AuNPs alone (A), 30 nm AuNPs in the presence of GB3 (B), and 30 nm AuNPs in the presence of BCA. The average values for three independently prepared samples support a small, but statistically significant, increase in the hydration radius when proteins are added to AuNP solutions. This increase is consistent with a single monolayer of compact protein on the AuNP surface (Table 2).
