Fig. 2. Evaluation of the CuAAC reactivity of ligands 1–3 using a fluorogenic CuAAC reaction. Reaction conditions: 100 μM 5, 50 μM 6, 100 μM CuSO4, 200 μM ligand, 500 μM sodium ascorbate (NaAsc) in 10/90 (v/v) methionine-free DMEM/PBS, room temperature. Yield was derived from the measured mean fluorescence intensity of 7 (Fig. S4†). Controls were performed without HPG. Error bars represent the standard deviation of data from three samples.
