Fig. 5. Illustration of the incorporation of HPG to proteins, CuAAC reaction in live cells, and subsequent hydrolysis of the biotinylated proteins to the amino acid derivative 10 for LC-ESI-MS/MS quantification. Reaction conditions: 100 μM 9, 100 μM CuSO4, 200 μM 1 or 3, 500 μM sodium ascorbate in 10/90 (v/v) methionine-free DMEM/PBS for 10 minutes. Color dots represent amino acid residues.
