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. 2017 Mar 24;3(3):e1601692. doi: 10.1126/sciadv.1601692

Fig. 2. An SPR assay recovers five independent biophysical/biochemical constants characterizing tethered enzymatic reactions by SHP-1.

Fig. 2

(A) Representative SPR trace (black dots) for SHP-1 injected over a surface immobilized with 48.5 RU of an ITIM peptide derived from LAIR-1 on a 28-repeat PEG linker (PEG28-ITIM). A fit of the MPDPDE model (red line) provides estimates for the indicated parameters. Early time data and fit are shown on the right. Unprocessed data are shown in fig. S1. (B) Anti-phosphotyrosine antibody injected at the end of the experiment shows reduced binding in the experimental flow cell (SHP-1) compared to a buffer-injected flow cell with equivalent peptide levels (control). (C) Schematic of reactions taking place when SHP-1 is injected over a surface of immobilized phosphorylated peptides. Note that peptide anchoring is displayed in one dimension for clarity, but because peptides are randomly coupled to a dextran matrix, which extends 100 to 200 nm above the surface, they are anchored in three dimensions.