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. 2017 Feb 16;6:64–69. doi: 10.1016/j.bonr.2017.02.002

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Cells were divided into five groups with each group treated with different ALE and PAM concentration (100 nM and 10 nM). (A): At day 21, calcium deposition was analysed. The results showed that BPs significantly stimulated hMSC mineralisation following drug treatment for 21 days when compared to cells that had been treated with OM only. (B): At day 14, extracellular collagen formation was analysed. The results showed that PAM significantly stimulated hMSC to produce collagen following treatment with drugs for 14 days when compared to cells that had been treated with OM only. No significant changes were observed in cells treated with ALE when compared to the control. (C): ALP activity after 7 days incubation was significantly increased in all cells treated with the lower dose of BPs drug when compared to the control group treated with OM only. Each column represents the mean ± SD.