Fig. 3.

(A) ALP activity. All dioxin-treated and antagonist-treated cells were cultured in osteogenic media. *p < 0.001 significance of ALP activity in dioxin-only treated cells relative to all other groups. (B) Matrix mineralization. Calcium deposition in the matrix of cells grown under standard or osteogenic conditions was visualized by Alizarin red staining, which was quantified using a cetylpyridinium chloride de-stain procedure. Note that all dioxin- and antagonist-treated cells were grown in osteogenic media. Columns, means from at least three independent experiments *p < 0.01, dioxin alone-treated wells vs. all other groups.