Effect of treatment with conditioned media, collected from differentiated Ocy454 cells (static and flow activated (2 dynes/cm2 for 24 h)), on C2C12 myofiber number and length. A) C2C12 myofiber number following treatment with OCY454 CM for 3 days and C) 6 days. B) C2C12 myofiber length following treatment with OCY454 CM for 3 days and D) 6 days. Data is Mean ± SEM. N = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs Vehicle. #p < 0.05 v Static. Data were analysed for statistical significance by ANOVA.