Figure 3. The RhopH2 interactome.
(a) Coomassie-stained SDS-PAGE gel of elution fractions from immune-precipitations performed with HA antibodies on lysates made from erythrocytes infected with RhopH2-HAglmS parasites at ring (R) or trophozoite stage (T). (b) Bar graph showing the total number of peptides of particular subclasses of proteins that were affinity purified with PfRhopH2-HA. (c) Pie charts showing the number of peptides from the respective RhopH proteins that affinity purified with RhopH2-HA. The numbers of peptides identified are indicated in brackets. Note RhopH1 includes all CLAG peptides. (d) Western blot of blue-native PAGE performed on erythrocytes infected with trophozoite stage RhopH2-HAglmS parasites that had been solubilized in either 0.25% Triton X-100 or 1% ASB detergent reveal RhopH2 is present in ~670 and ~410 kDa species. (e) Pie chart showing the numbers of the most abundant peptides from PEXEL proteins that affinity purified with PfRhopH2-HA from trophozoite stage parasites. (f) Pie chart showing the numbers of the most abundant peptides from host erythrocyte proteins that affinity purified with PfRhopH2-HA in ring stage parasites.