FIG 3.

PPMOs display synergy with traditional antibiotics. Three representative strains of P. aeruginosa (PAO1, M57-15, and W43532) were utilized to examine if PPMOs enhanced activity when combined with current traditional antibiotics. (a) Synergy isobolograms of PPMOs and antibiotics that target similar cellular processes. (b) Fractional inhibitory concentration (FIC) indices of PPMOs and antibiotics that target similar cellular processes. (c) FIC indices of PPMOs and antibiotics that target dissimilar cellular processes. FIC indices were calculated as described in Materials and Methods. An FIC index is considered synergistic when ≤0.5. Data represent the average of three to four independent experiments, and FIC indices were calculated from the best interaction point of these averages. Pip/Tazo, piperacillin-tazobactam; Scr, Scrambled.