FIG 3.
Confocal microscopy of L. donovani promastigotes treated with fluoresceinated AS-48. Promastigotes were incubated with fluoresceinated AS-48 (Fl-AS-48) at different concentrations. Incubation was carried out with 2 × 107 cells/ml for 4 h in HBSS-Glc. Promastigotes were stained with DAPI (10 μg/ml, 5 min) prior to their observation as unfixed parasites in confocal microscopy. Nomarski, differential interference contrast microscopy. Fluorescence settings: Fl-AS-48, λEXC = 488 nm, λEM = 519 nm; DAPI, λEXC = 358 nm, λEM = 461 nm. Bar, 10 μm.