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KN99α cells were treated with FLC, AMB or no. 54 at 0.19 to 50 μM (A) or no. 284 at 0.04 to 10 μM (B). (C to F) Following inhibition, 30-μl samples of the each of four replicates at 2×, 4×, and 8× the MIC were spotted on YPD agar and incubated for 48 h at 30°C.
