TABLE 3.
The best BLAST hits of orthologous enzymes in the sterol biosynthetic pathway presented in the genomes of 17 oomycetes, 3 algae, 2 fungi, and a diatoma
| Organism | Group | Subgroup | E value for enzyme in the sterol biosynthetic pathway |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ERG20 | ERG9 | ERG1 | ERG7 | ERG11 | ERG24 | ERG25 | ERG26 | ERG27 | ERG6 | ERG2 | ERG3 | ERG5 | ERG4 | |||
| Ca. albicans | Fungi | Ascomycota | 5.0E−155 | 1.0E−138 | 4.0E−143 | 0.0E+00 | 0.0E+00 | 3.0E−78 | 2.0E−118 | 1.0E−139 | 4.0E−111 | 8.0E−144 | 5.0E−66 | 6.0E−118 | 0.0E+00 | 7.0E−166 |
| As. fumigatus | Fungi | Ascomycota | 2.0E−118 | 8.0E−107 | 2.0E−89 | 0.0E+00 | 5.0E−147 | 2.0E−146 | 2.0E−100 | 2.0E−56 | 5.0E−36 | 1.0E−116 | 8.0E−52 | 3.0E−91 | 2.0E−171 | 2.0E−145 |
| Au. limacinum | Algae | Labyrinthulida | 3.0E−99 | 5.0E−65 | 4.0E−138 | 1.0E−73 | 4.0E−103 | 4.0E−17 | 9.0E−34 | 1.0E−08 | 5.0E−68 | 1.0E−62 | 6.0E−33 | 4.0E−53 | ||
| Au. anophagefferens | Algae | Pelagophyceae | 6.0E−94 | 1.0E−70 | 1.0E−129 | 6.0E−58 | 1.0E−73 | 1.0E−21 | 3.0E−25 | 4.0E−83 | 4.0E−10 | 1.0E−10 | 3.0E−102 | |||
| Ec. siliculosus | Algae | PX clade | 1.0E−118 | 8.0E−91 | 4.0E−65 | 4.0E−79 | 2.0E−66 | 2.0E−100 | 5.0E−09 | 1.0E−37 | 1.0E−99 | 1.0E−16 | 2.0E−17 | 3.0E−55 | ||
| Ph. tricornutum | Diatom | Bacillariophyta | 7.0E−115 | 1.0E−70 | 5.0E−126 | 3.0E−69 | 1.0E−100 | 6.0E−10 | 1.0E−33 | 2.0E−81 | 1.0E−19 | 1.0E−26 | 1.0E−52 | |||
| Ap. euteicheb | Oomycete | Saprolegniales | 3.0E−94 | 2.0E−69 | 4.0E−56 | 4.0E−66 | 8.0E−68 | 2.0E−73 | 1.0E−61 | 2.0E−25 | 2.0E−78 | 6.0E−16 | 1.0E−38 | |||
| Sa. parasitica | Oomycete | Saprolegniales | 9.0E−118 | 3.0E−79 | 4.0E−65 | 2.0E−158 | 1.0E−80 | 2.0E−84 | 2.0E−62 | 2.0E−50 | 5.0E−84 | 1.0E−62 | 1.0E−17 | 3.0E−50 | ||
| Py. insidiosumc | Oomycete | Pythiales | 6.0E−102 | 2.0E−11 | 2.0E−38 | 3.0E−16 | 3.0E−63 | 2.0E−17 | ||||||||
| Py. ultimum | Oomycete | Pythiales | 1.0E−119 | 6.0E−12 | 3.0E−36 | 8.0E−08 | 6.0E−18 | 2.0E−10 | 3.0E−79 | 3.0E−14 | 6.0E−26 | |||||
| Py. arrhenomanes | Oomycete | Pythiales | 6.0E−105 | 3.0E−14 | 1.0E−32 | 2.0E−19 | 7.0E−10 | 3.0E−17 | 2.0E−30 | |||||||
| Py. irregulare | Oomycete | Pythiales | 4.0E−123 | 4.0E−15 | 4.0E−40 | 1.0E−08 | 1.0E−14 | 9.0E−12 | 4.0E−81 | 3.0E−16 | 1.0E−29 | |||||
| Py. iwayamai | Oomycete | Pythiales | 2.0E−120 | 6.0E−17 | 4.0E−31 | 4.0E−07 | 5.0E−14 | 3.0E−10 | 1.0E−71 | 1.0E−15 | 4.0E−22 | |||||
| Py. vexans | Oomycete | Pythiales | 5.0E−95 | 1.0E−11 | 2.0E−36 | 4.0E−14 | 5.0E−12 | 1.0E−78 | 1.0E−14 | 1.0E−26 | ||||||
| Py. aphanidermatum | Oomycete | Pythiales | 6.0E−15 | 5.0E−35 | 6.0E−11 | 2.0E−19 | 6.0E−14 | 2.0E−81 | 9.0E−16 | 4.0E−31 | ||||||
| Ph. sojae | Oomycete | Peronosporales | 1.0E−118 | 3.0E−13 | 4.0E−38 | 4.0E−07 | 4.0E−12 | 9.0E−12 | 2.0E−82 | 2.0E−16 | 6.0E−29 | |||||
| Ph. ramorum | Oomycete | Peronosporales | 4.0E−119 | 2.0E−14 | 6.0E−38 | 3.0E−13 | 2.0E−11 | 7.0E−79 | 2.0E−16 | 5.0E−26 | ||||||
| Ph. parasitica | Oomycete | Peronosporales | 1.0E−115 | 4.0E−16 | 1.0E−39 | 2.0E−07 | 3.0E−14 | 4.0E−14 | 4.0E−79 | 3.0E−16 | 2.0E−26 | |||||
| Ph. capsici | Oomycete | Peronosporales | 1.0E−117 | 7.0E−12 | 8.0E−38 | 2.0E−08 | 9.0E−16 | 4.0E−14 | 8.0E−79 | 7.0E−14 | 1.0E−27 | |||||
| Ph. cinnamomi | Oomycete | Peronosporales | 4.0E−117 | 5.0E−16 | 4.0E−37 | 8.0E−07 | 7.0E−13 | 6.0E−10 | 3.0E−80 | 5.0E−18 | 2.0E−27 | |||||
| Ph. infestans | Oomycete | Peronosporales | 1.0E−115 | 2.0E−13 | 4.0E−39 | 9.0E−08 | 1.0E−14 | 2.0E−13 | 1.0E−74 | 3.0E−14 | 7.0E−28 | |||||
| Hy. arabidopsis | Oomycete | Peronosporales | 3.0E−105 | 2.0E−14 | 1.0E−12 | 4.0E−11 | 5.0E−14 | |||||||||
| Al. laibachii | Oomycete | Albuginales | 2.0E−107 | 2.0E−10 | 6.0E−16 | 4.0E−08 | 2.0E−11 | |||||||||
a
The best BLAST hits of orthologous enzymes in the sterol biosynthetic pathway are defined as having a cutoff E value of less than −6. The query sequences were derived from Saccharomyces cerevisiae.
b
Only transcriptome data were available and used for analysis.
c
Py. insidiosum (strain Pi-S) was isolated from a human patient with vascular pythiosis (19).