FIG 1.

Evaluation of small molecules for anti-CHIKV activity. (A) Small molecules were evaluated in Huh-7–CHIKV replicon cell culture. A 10 μM concentration of each compound was incubated with 5,000 cells/well in triplicate 96-well plates for 48 h at 37°C. The percent inhibition of the Renilla luciferase signal (± the standard deviation [SD]) was calculated as normalized to that in no-compound control lanes. The scatter plot represents percent inhibition data for 631 compounds. Only one compound, namely, β-d-N⁴-hydroxycytidine (NHC; indicated with a black arrow), was found to inhibit the replicon by over 80%. (B) Chemical structure of the NHC compound.