FIG 3.

Expression of recombinant MRP8 in LLC-PK1 cells. (A) RNA expression of recombinant human ABCC11 gene in LLC-PK1 ABCC11-overexpressing cells was significantly higher (left bar) than that of the parental cells (LLC-PK1). Data are shown as the ratio of ABCC11 gene expression relative to that of beta-actin gene expression determined by real-time PCR as described in Materials and Methods. Values are the means ± standard deviations from three independent experiments. Error bars represent standard errors from three independent experiments. Statistical significance was assessed by a two-way ANOVA multiple comparison test assuming equal variance (***, P value < 0.0001). (B) Human overexpressed MRP8 protein in LLC-PK1-ABCC11 and LLC-PK1 parental cells. Immunofluorescence staining of MRP8 protein, β-actin, and DAPI in both cell types was described in Materials and Methods. Photos were taken under the EVOS-II imaging station at a magnification of ×1,000.