. 2017 Mar 24;61(4):e01725-16. doi: 10.1128/AAC.01725-16

TABLE 1.

Effect of MRP8 overexpression on cytotoxicity of tenofovir in LLC-PK1 cells

Compound	CC₅₀ (μM) in:^a		Fold change (CC_50MRP8/CC_50WT)^c
Compound	WT cells^b	MRP8-overexpressing cells	Fold change (CC_50MRP8/CC_50WT)^c
Tenofovir	33,694 ± 839	161,076 ± 5,478	4.78
Tenofovir + 50 μM MK-571	34,938 ± 770	138,115 ± 976	3.95
Tenofovir + 100 μM MK-571	33,530 ± 466	10,713 ± 132	0.32

Values represent the means ± standard deviations of five independent experiments. The calculation was fitted to Richard's five-parameter dose-response curve (45) (asymmetric sigmoidal, with robust fit). See Materials and Methods for details.

WT, wild type, LLC-PK1 parental cells.

For all compounds there was a significant (P < 0.0001) decrease in toxicity due to MRP8 overexpression based on two-way ANOVA multiple comparison tests assuming equal variance.