FIG 4.

Effect of increased artesunate duration in combination with amodiaquine. In combination with a single dose of amodiaquine (Amq), three doses of 50 mg/kg of artesunate (AS) were administered at either 0, 8, and 20 h or 0, 24, and 48 h to female BALB/c mice. Time zero corresponds to a parasitemia of ∼10%, as determined by the percentage of infected erythrocytes in Giemsa-stained blood films. (A and B) Single doses of Amq at 6 mg/kg (A) or 12 mg/kg (B) were administered alone and in combination with AS. (C to E) Single doses of Amq at 18 mg/kg (C), 24 mg/kg (D), or 42 mg/kg (E) were administered in combination with AS. The data are represented as means ± SEM (or individual mice when group survival varied) and were analyzed by two-way ANOVA. (F) Time of recrudescence data summarized for all five trials and analyzed using an unpaired t test. ***, P < 0.001; **, P < 0.01; *, P < 0.05.