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TEM of N. caninum tachyzoites grown in HFFs and treated with BKI-1553. Cultures remained untreated for 4 days (A) or were treated with 5 μM BKI-1553 for 2 days (B) or 6 days (C). The inset in panel C is a low-magnification view of the complex shown in panel C. Note the progressive buildup of multinucleated complexes. Bars, 2.6 μm (A and B), 0.5 μm (C), and 4.8 μm (inset). Note the progressive buildup of multinucleated complexes in BKI-1553-treated cultures.
