FIG 5.

TEM of N. caninum tachyzoites treated with BKI-1507. Parasites were cultured in HFFs and treated with 5 μM BKI-1517 for 2 days (A to C), 4 days (D), and 6 days (E). (A to C) After 2 days of treatment, only vacuoles containing one or two parasites, albeit slightly enlarged, were found, and the PV matrix appears rather granular, with some of them exhibiting large vacuolization (C). (D and E) At 4 days of treatment (D), and especially at 6 days (E), parasites show clear signs of structural deterioration and are often embedded in a dense matrix. Bars, 0.7 μm (A and B), 0.9 μm (C and D), 0.5 μm (E). Insets in panels A to C represent the respective low-magnification views (bars, 4.8 μm).