FIG 6.

Parasite loads in brains of mice treated with BKI-1553 and the respective survival curves for offspring during 30 days p.p. Female BALB/c mice were mated, infected with Nc-Spain7 tachyzoites, and subsequently treated with BKI-1553 as detailed in Materials and Methods. Nonpregnant mice were euthanized at 3 weeks p.i., and dams were euthanized at 30 days p.p. (and thus at 44 days p.i.). (A and B) After euthanasia, brains were collected, and the tachyzoite load was quantified by real-time PCR and is presented as box plots. (A) Nonpregnant mice; (B) dams. The significance levels (obtained after a Kruskal-Wallis test) are indicated. (C and D) Survival curves of pups during 30 days p.p. with BKI-1553 (1553) (dashed line)- and corn oil (placebo) (solid line)-treated dams. As a parameter for survival, the Kaplan-Meier estimator (KME) is shown. For both treatment schedules, the differences in BKI-1553 and placebo survival curves were highly significant (P < 10⁻¹¹ [C] and P < 10⁻¹⁰ [D] by a log rank test).