FIG 7.

Parasite loads in brains of mice treated with BKI-1517. Female BALB/c mice were mated, infected with Nc-Spain7 tachyzoites, and subsequently treated with BKI-1517 as detailed in Materials and Methods. Nonpregnant mice were euthanized at 3 weeks p.i., and dams were euthanized at 30 days p.p. (and thus at 44 days p.i.). (A and B) After euthanasia, brains were collected, and the amount of tachyzoites was determined by quantitative PCR and are presented as box plots. (A) Nonpregnant mice; (B) dams. The significance levels for comparisons of the treated groups with the control group (obtained after a Kruskal-Wallis test followed by a pairwise Wilcoxon test) are indicated. (C and D) Survival curves of pups during 30 days p.p. of dams treated with BKI-1517 at 20 mg/kg (20) or 10 mg/kg (10) or with corn oil alone (0) for 6 days. As a parameter for survival, the Kaplan-Meier estimator (KME) is shown. For both treatment schedules, the differences in treated and placebo survival curves were highly significant (P < 10⁻⁴ for 10 mg/kg and P < 10⁻¹⁴ for 20 mg/kg by a log rank test).