TABLE 3.

Effects of BKI-1517 treatment on mortality, fertility, and cerebral Nc-Spain7 infection in nonpregnant mice, dams, and pups^c

Expt	Formulation and duration	No. of mice/total no. of mice			Litter size (no. of pups/no. of dams)	No. of pups/total no. of pups
		Seropositive	Nonpregnant mice, N. caninum brain positive	Dams, N. caninum brain positive		Neonatal mortalitya	Postnatal mortalityb	N. caninum brain positive
I	0 mg/kg for 6 days	16/16	5/6	10/10	56/10	1/56	55/55	55/55
	50 mg/kg for 6 days	16/16	0/6***	2/10***	24/10+	24/24***
II	0 mg/kg for 6 days	16/16	6/6	10/10	38/10	0/38	38/38	38/38
	10 mg/kg for 6 days	16/16	5/6	10/10	51/10	0/51	49/51	50/51
	20 mg/kg for 6 days	16/16	2/5*	8/11	60/11	0/60	13/60***	23/60***
	Not infected	0/8	0/8	0/8	48/8	0/48	0/48

^aProportion of pups born dead or that died within the 2 first days postpartum.

^bProportion of pups dead from days 3 to 30 p.p. (considered N. caninum positive).

^cBALB/c mice were treated with BKI-1553, infected with Nc-Spain7, or neither treated nor infected and euthanized as described in Materials and Methods. Adults and surviving pups were tested for the presence of N. caninum in their brains by real-time PCR. Pups that had died before the end of the experiment were considered N. caninum positive. The respective numbers of animals in the treated and placebo groups were compared by chi-square tests (⁺, P < 0.1; *, P < 0.05; **, P < 0.01; ***, P < 0.001).