FIG 7.

(A) Release of IL-6 and IL-8 by MDMs in the presence of LPS (10 μg/ml) or ionomycin (1 μM) compared with that by GaNP-treated MDMs exposed to the same agents. Preloading with GaNP significantly reduced the levels of IL-6 (*, P < 0.01) and IL-8 (*, P < 0.05) generation induced by LPS and ionomycin. (B) Western blot analysis of IKK-β. The overexpression of IKK-β was observed in the presence of GaNP. (C) Western blot analysis of NF-κB. The downregulation of NF-κB was observed in the presence of GaNP, confirming the relation with IKK-β. Statistically significant differences were determined using Student's t test (n = 3). *, P < 0.05 compared with non-drug-treated MDMs. a.u., arbitrary units.