Skip to main content
. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Virology. 2017 Feb 17;505:23–32. doi: 10.1016/j.virol.2017.02.008

Figure 1. Influenza cRNPs are exported from the nucleus.

Figure 1

(A) MDCK cells infected with WSN at a MOI of 2 were fractionated at indicated time points after infection. Markers for nucleus (lamin A/C) and cytosol (tubulin), together with viral NP and M1 proteins were detected by Western blotting using specific antibodies. (B) The specificity of qRT-PCR primer sets was determined using WSN NA vRNA (red), cRNA (yellow), and mRNA (green) templates prepared in vitro. The specificity of primers for WSN NAvRNA, NAcRNA, and NAmRNA is presented in respect to the percentage of its corresponding RNA template. (C) MDCK cells infected with WSN were fractionated, and total RNAs were extracted and applied for strand-specific real-time qRT-PCR. Quantities of vRNA, cRNA and mRNA of the NA segment of WSN influenza A virus in the nuclear (blue) and cytosolic (red) fractions are shown as averages with standard deviations from six independent experiments.