Fig. 5.

Bilirubin induces activation of M1 and M2 microglia. a Time course of mRNA expression levels of M1 and M2 microglia markers. WT and Ugt1 ^-/- mice mRNA cerebellar expression levels of CD68, CD86, MCR1, Arg1 and MCR2 were analysed at the indicated time points by qRT-PCR. For each gene, data were normalized according to the values of the WT samples at P5. For all the experiments, values represent the mean ± SD. Two-way ANOVA test, *p < 0.05. The number of WT and Ugt1 ^-/- was ≥3 in all the experiments and time points. b Representative IF of cerebellar sections from WT and Ugt1 ^-/- mice at P5 using an anti-MRC1 antibody (red) to detect M2 microglia, co-stained with an anti-Iba1 antibody (green) to highlight microglia. c Representative IF of cerebellar sections at P10 using an anti-CD68 antibody (red) to detect M1 microglia, co-stained with an anti-Iba1 antibody (green). For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 μm