Fig. 7.

Bilirubin-induced oxidative stress response. a Relative mRNA expression of different genes to assess the oxidative stress response. Expression levels of Nrf2 and HO1 in WT and Ugt1 ^-/- mice were analysed at P5, P8 and P10 by qRT-PCR. For each gene, data were normalized according to the values of the WT samples at P5. Values represent the mean ± S.D. Two-way ANOVA test, **p < 0.01. The number of WT and Ugt1 ^-/- was ≥3 in all the experiments and time points. b WB analysis and quantification of total cerebellum protein extracts of WT and Ugt1 ^-/- mice using an anti-HO1 antibody at the indicated time points. Actin was used as loading control. Student’s t test, *p < 0.05, **p < 0.01. c Representative IF of cerebellar sections from WT and Ugt1 ^-/- mice at P10 using an anti-HO1 antibody (red), co-stained with (left panel) an anti-calbindin antibody (green) to highlight Purkinje cells, (central panel) anti-NeuN antibody to highlight granule cells or (right panel) anti-Iba1 antibody to highlight microglia. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 μm