Fig. 6.

MS#30 and NMO#53 cause cytotoxicity to targeted cells via complement activation. PLP-eGFP slices were treated with rAb + HC (a–l) or rAb + C5-depleted HC (m–x) for 8 h and then stained for MAC and C3d deposition, respectively. Deposits of MAC (red) or C3d (red) were detected along PLP-eGFP+ oligodendrocyte processes and co-localized with MS#30 rAb (blue) in MS#30 + HC treated slices (a–d, m–p; arrows); whereas in NMO#53 + HC treated slices (e–h, q–t), MAC was co-localized with NMO#53 rAb (blue), which specifically labels astrocytes and end-foot processes (arrows), but was absent from oligodendrocyte processes (arrow heads). MAC and C3d staining were notably increased in MS#30 + HC or NMO#53 + HC treated slices when compared to Iso + HC treated ones (i–l, u–x). Scale bar: 25 μm