Table 3.

PCR conditions for genotyping of IL-4 gene position −590 (C>T).

Steps	Temperature (°C) and cycles		Time (sec)
Denature template	96		60
First initial denaturation	95	10 cycles	15
First annealing	65		50
First extension	72		40
Second initial denaturation	95	20 cycles	50
Second annealing	59		50
Second extension	72		50
Final extension	72		7 min
Incubation	4		5 min