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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Eur J Immunol. 2016 Sep 21;46(11):2650–2658. doi: 10.1002/eji.201646353

Figure 2. Aging increases the accumulation of ABCs that exhibit memory and activation phenotypes.

Figure 2

A: The spleens of young (2–4 months of age), aged (16–18 months of age), and advanced aged mice (22–24 months of age) were harvested and stained with appropriate fluorescent antibodies, and data were acquired via flow cytometry. Absolute cell number or proportions are shown. *, P < 0.01, n = 4–6 mice/group.

B: Enriched splenic B cells from non-transplanted and non-treated young and aged mice were stained with the indicated fluorescent antibody and gated on the ABC subpopulation. Relative median fluorescence intensity (MFI) is normalized to average MFI of young mice. *P<0.01,(t-test) between young and aged groups, n = 16 mice/group.

C: Enriched splenic B cells were obtained 12 days after skin allotransplantation and treatment with anti-CD45RB and anti-CD154 in young and aged recipient mice. Staining is per B. *, P < 0.01, (t-test) n = 6 mice / group.

D: Representative flow cytometry plots gated on ABC subpopulation from non – transplanted mice from B are shown (Gating is shown in Supplemental Figure 2A).