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. Author manuscript; available in PMC: 2017 Mar 27.
Published in final edited form as: Oncogene. 2016 Sep 26;36(13):1770–1778. doi: 10.1038/onc.2016.353

Figure 5.

Figure 5

Pancreatic fibroblasts upregulate and secrete miR-146a and Snail during gemcitabine treatment. (a) RT-PCR. miR-146a and Snail levels were altered in CAF1s during 1 µM GEM treatment (GT) (3 days) compared to untreated control (NT). (b) RT-PCR. CAF1s were treated with Snail-siRNA, and Snail and miR-146a expression was measured compared to negative siRNA control treated CAFs. (c) Exosomes from untreated and 1µM GEM-treated CAF1s were isolated and Snail mRNA and miR-146a within CAF1 exosomes was quantified via RT-PCR using relative Ct values. (d–e) L3.6 cells were treated with GT-CAF1 exosomes for 6 days (GT-CAF1/L3.6) or left untreated (L3.6 control). AsPC1 cells were treated with GT-CAF1 exosomes for 6 days (GT-CAF1/AsPC1) or left untreated (AsPC1 control). Snail (d) and miR-146a (e) levels were quantified in recipient cells via RT-PCR. *p-value<0.05; **p-value<0.01