Table 3.

Supernatants from splenocytes isolated from UVB-treated animals have no effect, while those from Sham-irradiated animals stimulate IFNγ production in normal mouse splenocytes.^#

Final Supernatant Concentration	Splenocyte Supernatant (Sham-treated)	Splenocyte Supernatant (UVB-treated)	Statistical Analysis
%	IFNγ, pg/mL		P value
0	669 ± 20	570 ± 47	0.221
3.125	863 ± 72	636 ± 48	0.026
6.25	852 ± 39	574 ± 28	0.046
12.5	1,091 ± 49	684 ± 39	0.011
25	1,466 ± 13	789 ± 36	0.004

^#Supernatants from splenocytes stimulated by anti-CD3e MoAb for 48 h from 5 sham- and 5 UVB-treated mice (90 min survival time) were pooled together and added at the % indicated to the cultures of normal mouse spleens stimulated for 48 h with anti-CD3e MoAb for 48 h. Levels of IFNγ quantitated as described in detail in the Materials and Methods section. Data presented as Mean ± SEM of triplicate cultures; exact P values evaluated with ANOVA (SigmaStat, San Jose, CA).