Table 4.

Serum-derived factors from UVB-treated animals inhibit IFNγ production by mouse splenocytes cultured with (Anti-CD3e MoAb).^#

Splenocytes incubated with 0.1% serum	IFNγ, pg/mL
Sham-treated mice	64 ± 0.9
UVB-treated mice	8.1 ± 0.9*

^#Sera from 6 UVB-treated and 5 Sham-treated mice were harvested 90 min after irradiation and pooled together (condition-dependent) and added for 2 h at 0.1% by volume to cultures of normal mouse splenocytes treated either with PBS or anti-CD3e MoAb in triplicates and incubated for 48 additional hours. IFNγ levels in harvested supernatants were analyzed ELISA as described in the Materials and Methods section. Statistical differences were evaluated with Student’s t-test, where

^*P<0.05.