Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 26;36(13):1816–1828. doi: 10.1038/onc.2016.345

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

PMC Copyright notice

Activity of DOCK5 is repressed by myosin II contractility. (a) HeLa cells were transfected with control or DOCK5 shRNA expression vectors and cultured in puromycin to select for expression of the resistance marker present in the vector. Cells were treated with the myosin II inhibitor blebbistatin (50 μM) or vehicle for 12 h and examined by phase contrast microscopy. (b) Localization of DOCK5 (green) and paxillin (red) in control and DOCK5-kd cells treated with blebbistatin for the indicated times. Scale bar indicates 10 μm. (c) Control cells treated with blebbistatin for 0 or 4 h were labeled to detect DOCK5 (green) and Arp3 (red). Scale bar represents 10 μm. (d) Western blot analysis of control and DOCK5-knockdown cells treated with blebbistatin for 0, 1, 2 and 4 h to detect phosphorylated forms and total levels of p130Cas, paxillin and FAK.