Table 2.

Helical Content of T-Peptides Does Not Decrease upon Binding of TolC^a

peptide	helical content (%)
	peptide alone	TolC-bound peptide
T1–190	73 ± 6	77 ± 4
T1–140	37 ± 5	59 ± 8
T41–190	80 ± 8	83 ± 7

^aFar-UV CD spectra (185–260 nm) of 1 μM T-peptides, TolC trimer, and their complex at a 1:1 molar ratio were measured in cuvettes with optical path lengths of 0.1, 0.2, and 1.0 mm. Spectra of peptides and difference spectra of TolC-bound peptides (“TolC/T-peptide” – “TolC”) expressed in mean molar residue ellipticity units were analyzed using Selcon3, Contin, and K2D programs on the DichroWeb Internet site.⁴² The buffer consisted of 0.1 M sodium phosphate (pH 7.0) and 0.03% DDM.